RTUFirst Year (Common)Yr 2024 · Sem 22024

Q7Engineering Chemistry

Question

2 marks

Q.7 Define Beer-Lambert Law.

Answer

The combined Beer-Lambert Law mathematically asserts that the absolute amount of monochromatic light physically absorbed by a transparent chemical solution is strictly directly proportional to both the exact molar concentration of the absorbing chemical species and the total physical path length of the light.

The Beer-Lambert law is the absolute fundamental governing physical principle utilized strictly in all quantitative spectrophotometric chemical analysis.

  • = The absolute measured Absorbance (Optical Density).
  • = The exact incident light intensity.
  • = The exact transmitted light intensity.
  • = The molar extinction coefficient (or molar absorptivity), a strict constant for a specific molecule at a specific precise wavelength.
  • = The exact molar concentration of the absorbing solution.
  • = The absolute physical path length of the sample cell (cuvette), usually strictly 1 cm.
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